Hossein Daghigh Kia; Zahra Blouki
Volume 19, Issue 3 , November 2017, , Pages 727-738
Abstract
The aim of this study was to investigate the antioxidant effect of coenzyme Q10 on ram semen quality after the freezing-thawing process. Five Ghezel rams were used for sperm collection twice a week in five replicates. This experiment consisted of 5 treatments, coenzyme Q10 at four levels (0.5, 1, 2 and ...
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The aim of this study was to investigate the antioxidant effect of coenzyme Q10 on ram semen quality after the freezing-thawing process. Five Ghezel rams were used for sperm collection twice a week in five replicates. This experiment consisted of 5 treatments, coenzyme Q10 at four levels (0.5, 1, 2 and 2.5 μmol) and control group (without antioxidant). In this study, motility, viability, membrane integrity, abnormality parameters of sperm, malondialdehyde, total antioxidant capacity, glutathione peroxidase and superoxide dismutase activity were measured following freeze-thawing. The results showed that the total motility in samples with 0.5 and 1 μM of coenzyme Q10 antioxidant was significantly higher than the control group (p< 0.05). Samples with 0.5 μM Coenzyme Q10 had the lowest percentage of abnormal sperm and lipid peroxidation level compared to the control group (p< 0.05). Viability, plasma membrane integrity and total motility were better in all treated groups compared to the control group (p< 0.05). The VCL, VSL and VAP parameters were improved in groups receiving 0.5, 1 and 2 μM coenzyme Q10 compared with the other groups (p< 0.05). The group receiving 2.5 μM coenzyme Q10 had the best impact on the superoxide dismutase activity than other groups. The results of this study showed that adding 0.5 and 1 μM Coenzyme Q10 improved some of the sperm parameters after the freezing-cracking process.